Pseudobase++2.0 Instructions

Quick Search

 Select "All" from the drop down menu and the input field empty to see the whole dataset and related information

 To find PKB ID, NCBI ID, Pseudoknot span or any other related information to the pseudoknots select any of the following single input

  1. Abbreviation

    Select "Abbreviation" from the drop down menu and insert any abbreviations of different organisms in the input box, some examples are given below

    • BLV - Bovine Leukemia Virus
    • BWYV - Beet Western-Yellows Virus
    • EIAV - Equine Infectious Anemic Virus
    • FIV - Feline Immunodeficiency Virus
    • TMGMV_UPD-PK1 - tobacco mild green mosaic virus
  2. Classification
  3. Select "Classification" from the drop down menu and input the classification type based on hairpin loop (H) or Bulge loop (L). There could be other classifications as well such as
    eg. HHH
       HLin
       HLout

  4. Comments
  5. Select "Comments" from the drop down menu and input any keywords or comments related to a Pseudoknot
    eg. tRNA

  6. Continuous
  7. Select "Continuous" from the drop down menu and input "yes" or "no" for finding Pseudoknots that are continuous or discontinuous respectively.

    eg. yes
       no

  8. Definition
  9. Select "Definition" from the drop down menu and input any keywords or definition related to a Pseudoknot
    eg. ribosomal frameshift

  10. NCBI number
  11. Select "NCBI number" from the drop down menu and input any NCBI IDs to get the Pseudoknot
    eg. AF035200

  12. Keywords
  13. Select "Keywords" from the drop down menu and input any keyword to find the related Pseudoknot
    eg. tymoviruses

  14. Organism
  15. Select "Organism" from the drop down menu and input any names to find a Pseudoknot of that organism
    eg. human

  16. PKB number
  17. Select "PKB number" from the drop down menu and input any Pseudobase IDs to get the Pseudoknot
    eg. PKB100

  18. RNA type
  19. Select "RNA type" from the drop down menu and input any keyword to find the related Pseudoknot
    eg. Viral ribosomal frameshifting

  20. Span
  21. Select "Span" from the drop down menu and input any numeric value to find a Pseudoknot of that length
    eg. 121

  22. Submitted by
  23. Select "Submitted by" from the drop down menu and input any authors name to find the Pseudoknot submitted by that individual
    eg. A.P.Gultyaev

  24. Supported by
  25. Select "Supported by" from the drop down menu and input any keywords to find the related Pseudoknot
    eg. Sequence comparison

    Advanced Search

      Combinations of different desired properties will work with keeping the other parameters blank.
      Following shows the type of inputs required for each field.

    1. Sequence: First few molecules such as AAAC or full sequence
    2. Keyword: type any words
    3. PKB no.: PKB100
    4. Organism: Human
    5. Comment: tRNA
    6. Submitted by: A.P.Gultyaev
    7. Span: From the drop down menu select "greater than", "less than" or "equal to" and input the numeric value of the length. eg. 200
    8. RNA type: Select any type from the dropdown menu to find the related Pseudoknot.
    9. Supported by: Select any type from the dropdown menu to find the related Pseudoknot.
    10. Pseudoknot type: Select any type from the dropdown menu to find the related Pseudoknot.
    11. Loop length: From the drop down menu select "greater than", "less than" or "equal to" and input the numeric value of the length. eg. 10
    12. Stem length: From the drop down menu select "greater than", "less than" or "equal to" and input the numeric value of the length. eg. 10
    13. Continuous: to check only Psuedoknots without gaps "yes", with gaps "no" or use "both" to see all.
    14. Check any combination of the boxes to add those columns in the result table